The effects of caffeine on Salvia Morada

Idea: To treat the purple sage with different caffeine answers over a period of 4 weeks . One plant was self-aggrandizing healthy and unexposed; this was the control plant. One plant was grown in a solution containing caffeine tablets, ane with the energy make happy rubicund Bull and the other with coffee. Dosages and/or water solutions depended upon capability of the product. One caffeine tablet contains 200 mg of a caffeine, the average cup of coffee contains 70mg of caffeine and an 8oz can of Red Bull contains 80mg of caffeine.

Once the plants where treated, tissue paper haves where taken and observed under microscope. desoxyribonucleic acid examples where isolated using PCR methods and gel electrophoresis was performed in ensnare to observe banding battles with the control plant. This was in hopes of revealing a genetic affect of caffeine on the plant.

Hypothesis

In observing the tissue samples, I believed that there would be a difference in color and shape of the mesophyll, when compared to the controlled sample. I believed this will be referable to the dehydrating effects of caffeine, creating a lack of stroma or chlorophyll, thus bear upon photosynthesis an in turn, affecting color. I as well believe the sample with the energy drink will be healthiest among the exposed sample due to its sugar content.

I predicted that the banding results would reveal a difference in banding with the control sample, indicating genetic effect. I also believe this difference would be less prominent in the coffee sample.

Method & Materials

gives where treated daily with an 8oz solution. Plant As solution contained a crushed 200mg tablet dissolved in 8oz of water. Plant Bs solution contained 2oz Red Bull and 6oz water. Plant Cs solution contained one tablespoon of instant coffee in 8 oz of water.

Growth say and color where monitored.

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